Annals of Diagnostic Pathology
Volume 10, Issue 1 , Pages 36-38, February 2006

Detection of TLS/FUS-CHOP fusion transcripts in a case of oral liposarcoma

  • Elena Riet Correa Rivero, MDS, PhD

      Affiliations

    • Department of Oral Pathology, School of Dentistry, University of São Paulo, 05508-900 São Paulo, Brazil
    • ,
  • Ricardo Alves Mesquita, PhD

      Affiliations

    • Department of Oral Pathology, School of Dentistry, University of Minas Gerais, 31270-901 Belo Horizonte, Brazil
    • ,
  • Suzana Cantanhede Orsini Machado de Sousa, PhD

      Affiliations

    • Department of Oral Pathology, School of Dentistry, University of São Paulo, 05508-900 São Paulo, Brazil
    • ,
  • Fabio Daumas Nunes, PhD

      Affiliations

    • Department of Oral Pathology, School of Dentistry, University of São Paulo, 05508-900 São Paulo, Brazil
    • Corresponding Author InformationCorresponding author. Tel.: +55 11 3091 7902; fax: +55 11 3818 7894.

Abstract 

Objective

The aim of this study was to detect the chromosomal translocation t(12;16)(q13;p11) that leads to a gene fusion encoding a FUS-CHOP chimeric protein and has been shown to be highly characteristic of myxoid and round cell subtypes of liposarcoma, in a case of oral myxoid liposarcoma.

Method and Materials

Nested reverse transcriptase–polymerase chain reaction to detect the TLS/FUS-CHOP fusion gene transcript was performed. A case of inflammatory fibrous hyperplasia and a case of oral lipoma were included as negative controls.

Results

Only the myxoid oral liposarcoma showed a 103–base pair product, specific of TLS/FUS-CHOP fusion type II transcript.

Conclusion

The identification of FUS-CHOP transcript is potentially useful in the diagnosis and research of oral liposarcomas.

Keywords: Chromosomal translocation, Cancer development, FUS-CHOP chimeric protein, Liposarcomas, RT-PCR, TLS/FUS-CHOP fusion gene

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PII: S1092-9134(05)00047-X

doi:10.1016/j.anndiagpath.2005.04.006

Annals of Diagnostic Pathology
Volume 10, Issue 1 , Pages 36-38, February 2006

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