αB-crystallin: A novel marker of invasive basal-like and metaplastic breast carcinomas

Sitterding, Stephanie M.; Wiseman, William R.; Schiller, Carol L.; Luan, Chunyan; Chen, Feng; Moyano, Jose V.; Watkin, William G.; Wiley, Elizabeth L.; Cryns, Vincent L.; Diaz, Leslie K.

doi:10.1016/j.anndiagpath.2007.02.004

« Previous Next »Annals of Diagnostic Pathology
Volume 12, Issue 1 , Pages 33-40, February 2008

αB-crystallin: A novel marker of invasive basal-like and metaplastic breast carcinomas

Some of the results were presented in poster format at the 95th Annual Meeting of the United States and Canadian Academy of Pathology, February 2006, Atlanta, GA.

Stephanie M. Sitterding, MD
- Department of Pathology, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
William R. Wiseman, MD
- Department of Pathology, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
Carol L. Schiller, MD
- Department of Pathology, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
Chunyan Luan, MS
- Department of Pathology, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
Feng Chen, MD
- Cell Death Regulation Laboratory, Departments of Medicine and Cell and Molecular Biology, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
Jose V. Moyano, PhD
- Cell Death Regulation Laboratory, Departments of Medicine and Cell and Molecular Biology, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
William G. Watkin, MD
- Evanston Northwestern Healthcare, Evanston, IL 60201, USA
Elizabeth L. Wiley, MD
- Department of Pathology, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
Vincent L. Cryns, MD
- Cell Death Regulation Laboratory, Departments of Medicine and Cell and Molecular Biology, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
- They have contributed equally and share senior authorship.
Leslie K. Diaz, MD
- Department of Pathology, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
- They have contributed equally and share senior authorship.
- Corresponding author. Department of Pathology, Abbott Northwestern Hospital Laboratory, Minneapolis, MN 55407, USA.

published online 08 October 2007.

Abstract

Basal-like tumors are a newly recognized estrogen receptor (ER) negative and HER2 negative breast cancer subtype that express basal epithelial genes and are associated with poor survival. Metaplastic carcinomas are thought to belong within the basal-like group. We have recently demonstrated that the small heat shock protein αB-crystallin is commonly expressed in basal-like tumors and contributes to their aggressive phenotype. The current study examined the rates and patterns of αB-crystallin expression in whole tissue sections of human breast, including normal tissue, proliferative lesions, in situ and invasive carcinomas (ER positive, HER2 positive, basal-like, and metaplastic cancers). In normal breast tissue, proliferative lesions and in situ carcinomas, αB-crystallin expression was restricted to the myoepithelial cell compartment of ductal and lobular units. Most basal-like and metaplastic carcinomas demonstrated cytoplasmic expression of αB-crystallin (81% and 86%, respectively). Conversely, no staining for αB-crystallin was observed in nonbasal-like (ie, ER positive or HER2 positive) breast carcinomas. Taken together, our results indicate that αB-crystallin is a sensitive (81%) and specific (100%) marker for basal-like breast carcinomas. Moreover, the high rates of expression of αB-crystallin in metaplastic breast carcinomas (86%) suggest that these tumors may represent a histologically distinctive subset of basal-like breast tumors with a similar underlying molecular etiology.

Keywords: Breast, Breast cancer, Basal-like, Metaplastic, αB-crystallin