Annals of Diagnostic Pathology
Volume 12, Issue 6 , Pages 387-396 , December 2008

Histology without formalin?

References 

  1. Fox CH, Johnson FB, Whiting J, Roller PP. Formaldehyde fixation. J Histochem Cytochem. 1985;33(8):845–853
  2. DHHS (NIOSH): Current intelligence bulletin 34. Formaldehyde: evidence of carcinogenicity. DHHS (NIOSH), 1981; Publicat. No. 81-1-11, p. 12.
  3. Formaldehyde—Prioritization of toxic air contaminants—Oct. 2001; Children's Environmental Health Protection act. p. 16.
  4. In: Formaldehyde. OSHA Fact Sheet. 2002;p. 2
  5. Formaldehyde. OSHA Regs. (Standards-29 CFR) Standards 1910-1048.
  6. Formaldehyde (CASRN-50-00-0). Integrated Risk Information System US-EPA, IRIS Summaries (CAS Registry # 50-00-0), p. 16.
  7. Vaughn TL, Strader C, Davis S, Daling JR. Formaldehyde and cancers of the pharynx, sinus and nasal cavity: II. Residential exposure. Int J Cancer. 1986;38:685–688
  8. Vaughn JL, Strader C, Davis S, Daling JR. Formaldehyde and cancers of the larynx, sinus and nasal cavity: I. Occupational exposures. Int J Cancer. 1986;38:677–683
  9. Blair A, Stewart PA, Hoover RN, et al. Mortality among industrial workers exposed to formaldehyde. J Natl Cancer Inst. 1986;76(6):1071–1084
  10. Marsh GM, Youk AO, Buchani JM, et al. Pharyngeal cancer mortality among chemical plant workers exposed to formaldehyde. Toxicology & Industrial Health. 2002;18(6):257–268
  11. Gardner MJ, Pannett B, Winter PD, Cruddas AM. A cohort study of workers exposed to formaldehyde in the British chemical industry: an update. Br J Ind Med. 1993;50(9):827–834
  12. Pinkerton VE, Hein MJ, Stayner LT. Mortality among a cohort of garment workers exposed to formaldehyde: an update. Occupat Environm Med. 2004;61:193–200
  13. IARC classifies formaldehyde as carcinogenic to humans, 30 June 2004 (International Agency Research on Cancer). http://www.iarc.fr/ENG/Press
  14. Buesa RJ. In: Histology review. North Miami Beach (Fla): Sciences & Business Institute; 1996;p. 141
  15. Buesa RJ. Histology safety: now and then. Ann Diag Pathol. 2007;11(5):334–339
  16. Gray P. In: The microtomist's formulary and guide. New York: Blakiston; 1954;p. 794
  17. Bancroft JD, Stevens A: Theory and practice of histological techniques. Churchill Livingstone, London, 1977; viii + p. 436.
  18. Acton A, Harvey T, Grow MW. An examination of non-formalin based fixation methods for Xenopus embryos. Developm Dynam. 2005;233:1464–1469
  19. Battifora H. Quality assurance issues in immunohistochemistry. J Histotech. 1999;22(3):169–175
  20. Helander KG. Kinetic studies of formaldehyde binding in tissue. Biotech Histochem. 1994;69(3):177–179
  21. Helander KG. Formaldehyde binding in brain and kidney: a kinetic study of fixation. J Histotech. 1999;22(4):317–318
  22. Munakata S, Hendricks JB. Effect of fixation time and microwave oven heating time on retrieval of the Ki-67 antigen from paraffin-embedded tissue. J Histochem Cytochem. 1993;41(8):1241–1246
  23. Sompuran SR, Vani K, Messana E, Bogen SA. A molecular mechanism of formalin fixation and antigen retrieval. Am J Clin Pathol. 2004;121:190–199
  24. Helander KG, Widehn S, Helander HF. Kinetic studies of glutaraldehyde binding in liver. Biotech Histochem. 2002;77:207–212
  25. Boon ME, Kok LP. Formalin is deleterious to cytoskeleton proteins: do we need to replace it by formalin—free Kryofix?. Eur J Morphol. 1991;29(3):173–180
  26. Login GR, Dvorak AM: The microwave tool book. Beth Israel Hospital, Boston, Boston, Mass, 1994; viii + p. 164.
  27. Kok LP, Boon ME: Microwaves for the art of microscopy. Coulomb Press, Leyden, 2003; xvi + p. 368.
  28. Lee KY, Shibutami M, Inoue K, et al. Methacarn fixation—effects of tissue processing and storage conditions on detection of mRNAs and proteins in paraffin-embedded tissues. Analyt Biochem. 2006;351(1):36–43
  29. Login GR, Stavinoha WB, Dvorak AM. Ultrafast microwave energy fixation for electron microscopy. J Histochem Cytochem. 1986;34(3):381–387
  30. Werner M, Chott A, Fabiano A, Battifora H. Effect of formalin fixation and processing in immunohistochemistry. Ann J Surg Pathol. 2000;24(7):1016–1019
  31. Gillespie JW, Beat CJM, Bichsel VE, et al. Evaluation of non-formalin tissue fixation for molecular profiling studies. Am J Pathol. 2002;160(2):449–457
  32. Warmington AR, Wilkinson JM, Riley CB. Evaluation of ethanol-based fixatives as a substitute for formalin in diagnostic clinical laboratories. J Histotech. 2000;23(4):299–308
  33. Boon ME, Schmidt U, Cramer-Knjnenburg GY, van Krieken JH. Using Kryofix As alternative for formalin results in more optimal and standardized immunostaining of paraffin sections. Pathol Res Pract. 1992;188(7):832–835
  34. Prentø P, Lyon H. Commercial formalin substitutes for histopathology. Biotech Histochem. 1997;72(5):273–282
  35. Delfour C, Roger P, Bret C, et al. RCL2, a new fixative, preserves morphology and nucleic acid integrity in paraffin-embedded breast carcinoma and microdissected breast tumor cells. J Mol Diag. 2006;8(2):157–169
  36. Cox ML, Scharay CL, Luster CN, et al. Assessment of fixatives, fixation, and tissue processing on morphology and RNA integrity. Exp Mol Pathol. 2006;80:183–191
  37. Mitchell F, Ibrahim S, Gusterson BA. Improved immunohistochemical localization of tissue antigens using modified methacarn fixation. J Histochem Cytochem. 1985;33(5):491–495
  38. Puchter H, Waldrop FS, Meloan SN, et al. Methacarn (methanol-Carnoy) fixation. Histochem Cell Biol. 1970;21(2):97–116
  39. Shibutami M, Uneyama C, Miyazaki K, et al. Methacarn fixation: a novel tool for analysis of gene expressions in paraffin-embedded tissue specimens. Lab Invest. 2000;80:199–208
  40. Vincek V. Word of caution: a comment on the article by Cox et al “Assessment of fixatives, fixation, and tissue processing on morphology and RNA integrity”. Exp Mol Pathol. 2005;Epnb ahead of print. Exp Mol Pathol, 2006; 81(2):181
  41. Hannouche D, Raould A, Nizard RS, et al. Embedding of bone samples in methylmethacrylate: a suitable method for tracking LacZ mesenchymal stem cells in skeletal tissues. J Histochem Cytochem. 2007;55(3):255–262
  42. Wester K, Asplund A, Bäckvall H, et al. Zinc-based fixative improves preservation of genomic DNA and proteins in histoprocessing of human tissues. Lab Invest. 2003;83:889–899
  43. Ben-Ezra J, Johnson DA, Rossi J, et al. Effect of fixation on the amplification of nucleic acids from paraffin-embedded material by the polymerase chain reaction. J Histochem Cytochem. 1991;39(3):351–354
  44. Titford ME, Horenstein MG. Histomorphologic assessment of formalin substitute fixatives for diagnostic surgical pathology. Arch Pathol Lab Med. 2005;129:502–506
  45. Srinivasan M, Sednak D, Jewell S. Effect of fixatives and tissue processing on the content and integrity of nucleic acids. Am J Pathol. 2002;161(6):1961–1971
  46. Greer CE, Lund JK, Manos MM. PCR amplification from paraffin-embedded tissues: recommendations on fixatives for long-term storage and prospective studies. PCR Methods Appl. 1991;1:46–50
  47. Weiss LM, Chen YY. Effects of different fixatives on detection of nucleic acids from paraffin-embedded tissues by in situ hybridization using oligonucleotide probes. J Histochem Cytochem. 1991;39(9):1237–1242
  48. Vincek V, Nassiri M, Nadji M, Morales AR. A tissue fixative that protects macromolecules (DNA, RNA, and protein) and histomorphology in clinical samples. Lab Invest. 2003;83(10):1427–1435
  49. Nadji M, Nassiri M, Vincek V, et al. Immunohistochemistry of tissue prepared by a molecular-friendly fixation and processing system. Appl Immunohistochem Mol Morphol. 2005;13:277–282
  50. Hassell J, Hand AR. Tissue fixation with diimidoesters as an alternative to aldehydes. I. Comparisons of cross-linking and ultrastructure with dimethyl suberimidate and glutaraldehyde. J Histochem Cytochem. 1974;22(4):223–239
  51. Majewski P, Pernak A, Grzymislawski M, et al. Ionic liquids in embalming and tissue preservation. Can traditional formalin fixation be replaced safely?. Acta Histochem. 2003;105(2):135–142
  52. Pernak A, Iwanik K, Majewski P, et al. Ionic liquids as an alternative to formalin in histopathological diagnosis. Acta Histochem. 2005;107(2):149–156
  53. Meyer R, Niedobitek F, Wenzelides K. Experiences with NoTox formalin substitute. Pathologe. 1996;17(2):130–132
  54. Dapson RW. Glyoxal fixation: how it works and why it only occasionally needs antigen retrieval. Biotech Histochem. 2007;82(3):161–166
  55. Trpkov K, Renault P, Yilmaz A, Ali-Ridha N. IBF as a formalin substitute fixative in prostate biopsy pathology. Arch Pathol Lab Med. 2006;130(1):13–14
  56. Nace EK, Steurer FJ, Eberhard ML. Evaluation of Streck tissue fixative, a nonformalin fixative for preservation of stool samples and subsequent parasitologic examination. J Clin Microbiol. 1999;37(12):4113–4119
  57. Rosai J. Why microscopy will remain a cornerstone of surgical pathology. Lab Invest. 2007;80:403–408
  58. Mason JT, O'Leary TJ. Effects of formaldehyde on protein secondary structure: a colorimetric and infrared spectroscopic investigation. J Histochem Cytochem. 1991;39(2):225–229
  59. Ostrowski K, Komender J, Kwarecki K. Quantitative investigations on the solubility of proteins extracted from tissues fixed by different chemical and physical methods. Cell Molec Life Sci. 1961;17(4):183–184
  60. Eltoum I, Fredenburgh J, Myers RB, Grizzle WE. Introduction to the theory and practice of fixation of tissues. J Histotech. 2001;24(3):173–190
  61. Hopwood D. Fixatives and fixation: a review. Histochem J. 1969;1:323–360
  62. Sliders W, Hopwood D. Buffered phenol formaldehyde (pH 7.0 and pH 5.5): improved fixation in an enclosed tissue processor. Med Lab Sci. 1989;46(1):74–76
  63. Leonard JB, Shepardson SP. A comparison of heating modes in rapid fixation technique for electron microscopy. J Histochem Cytochem. 1994;42(3):383–391
  64. Selvarajan S, Bay BH, Choo A, et al. Effect of fixation period on HER2/neu gene amplification detected by fluorescence in situ hybridization in invasive breast carcinoma. J Histochem. 2002;50(12):1693–1696
  65. MnTAP: Formalin recovery in Health Care labs. Minnesota Technical Assistance Program (MnTAP) Fact Sheet. http://www.mntap.umn.edu
  66. Formaldehyde (methyl aldehyde). Australian Government. 2007;
  67. WHO . Formaldehyde and safety IPCS International programme on chemical safety. Health and safety guide no. 57. Geneva: WHO; 1991;
  68. Goyer N, Bégin D, Beaudry C, et al. In: Prevention guide. Formaldehyde in the workplace. Canada: IRS ST—Institut de Recherche Robert-Sauvé en Santé et en Sécurité du travail; 2006;p. 55
  69. Reglur um mengunarmök og aðgerðir til að draga úr mengun á vinnustöðum. Stjtd.B19uv. 154/1999.
  70. Norm GN2.2.5.1313.03 (Resolution of the State Head Sanitary Physician of the Russian Federation) Government Sanitary and Epidemiologic Service of Russia—30 April 2003.

PII: S1092-9134(08)00081-6

doi: 10.1016/j.anndiagpath.2008.07.004

Annals of Diagnostic Pathology
Volume 12, Issue 6 , Pages 387-396 , December 2008